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  • Title: [Analysis of cytoplasmic aspartate aminotransferase using active enzyme centrifugation].
    Author: Chebotareva NA, Pavlenko AA, Kurganov BI.
    Journal: Biokhimiia; 1984 May; 49(5):870-4. PubMed ID: 6743708.
    Abstract:
    Using active enzyme centrifugation, it was demonstrated that pig heart cytoplasmic aspartate aminotransferase retains its dimeric conformation with a decrease in protein concentration down to 0.2 microgram/ml (50 mM L-aspartate, 5 mM alpha-ketoglutarate, 0.1 M NaCl, 0.05 M potassium-phosphate buffer, pH 7.4, 20 degrees C).
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