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  • Title: The packaging initiation site of phage P22. Analysis of packaging events by transduction.
    Author: Kufer B, Backhaus H, Schmieger H.
    Journal: Mol Gen Genet; 1982; 187(3):510-5. PubMed ID: 6757663.
    Abstract:
    P22 lysates were grown on Salmonella strains carrying P22 prophages deleted to various extents. Transducing bacterial markers at both sides of the prophage insertion site it could be shown that: (i) transduction of markers can be enhanced by the prophage pac site; (ii) the recognition signal pac is in the area of gene 3 on the phage genome and thus close to the cutting site(s); (iii) transposon Tn10 may also act as a signal for packaging initiation; (iv) (at least) Tn10 initiates packaging sequences in both directions.
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