These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Localization of high molecular weight microtubule-associated proteins (MAP1 and MAP2) in a HeLa microtubule-organizing centre.
    Author: Mascardo RN, Sherline P, Weatherbee J.
    Journal: Cytobios; 1982; 35(138):113-27. PubMed ID: 6761077.
    Abstract:
    With a rabbit antiserum specific for high molecular weight microtubule-associated proteins (HMW/MAPs: MAP1 and MAP2), centrosomes in interphase and mitotic HeLa cells were visualized by indirect immunofluorescence. Pre-immune serum did not stain a similar organelle. Microtubules were observed to originate from the centrosomes, demonstrating their microtubule organizing (MTOC) function. The MTOC antigen(s) to which the anti-HMW/MAP antiserum reacted was resistant to Colcemid, Triton-X-100, Nonidet P-40 and Brij 35. Immunofluorescence localization of the MTOCs was blocked by pre-incubation of the antiserum with a MAP1--MAP2 preparation but not with tubulin, actin, or a 58 K intermediate filament protein.
    [Abstract] [Full Text] [Related] [New Search]