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  • Title: Three-step purification of retinol-binding protein from rat serum.
    Author: McGuire BW, Chytil F.
    Journal: Biochim Biophys Acta; 1980 Feb 27; 621(2):324-31. PubMed ID: 6766325.
    Abstract:
    Rat serum retinol-binding protein has been purified to apparent homogeneity in high yield by a new procedure utilizing three simple steps: DEAE-cellulose chromatography at pH 6.0, Sephadex G-75 gel filtration in the presence of 3.0 M urea, and finally DEAE-cellulose chromatography at pH 8.3. The second step accomplished the dissociation and separation of retinol-binding protein from its complex with prealbumin; this represents a substantial improvement over published procedures, in which sample recycling and preparative polyacrylamide gel electrophoresis were necessary. The purified retinol-binding protein was characterized by molecular weight measurement, fluorescence spectra and immunological properties.
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