These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Antibody potential of human peripheral blood lymphocytes differentially expressing surface membrane IgM.
    Author: Thiele CJ, Stevens RH.
    Journal: J Immunol; 1980 Apr; 124(4):1898-904. PubMed ID: 6767777.
    Abstract:
    The appearance of circulating pokeweed mitogen-reactive B lymphocyte subpopulations responsible for the synthesis of IgM and IgG anti-tetanus toxoid antibody after in vivo booster immunization has been analyzed for differential expression of surface membrane IgM. B cells were rosetted with human mu-chain specific antiglobulin-coupled ox red blood cells and separated by density centrifugation into mu+ and mu- populations. After 8 days in vitro culture with irradiated T cells and pokeweed mitogen, antibody synthesis was assessed by quantitative radioimmunoassay. Mu+ cells synthesized both IgG and IgM anti-tetanus toxoid antibodies, whereas mu- cells predominantly synthesized IgG anti-tetanus toxoid antibodies. Limiting dilution analysis revealed a greater number of IgG anti-tetanus toxoid precursors in the mu+ population at all times after immunization compared with mu- cells. However, the activity, as measured by the amount of antibody produced per IgG anti-tetanus toxoid antibody precursor, was 2 to 4 times greater in the mu- population than that in the mu+ population. These results indicate that functionally distinct human circulating B lymphocytes can be identified by their differential expression of surface membrane IgM.
    [Abstract] [Full Text] [Related] [New Search]