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  • Title: A sensitive, single-radial diffusion autoradiographic zone size enhancement technique for the assay of influenza haemagglutinin.
    Author: Wood JM, Schild GC, Newman RW.
    Journal: J Gen Virol; 1980 Apr; 47(2):355-63. PubMed ID: 6767808.
    Abstract:
    A novel radial-diffusion technique in agarose gels is described which is applicable to the assay of small concentrations of influenza haemagglutinin (HA) antigen. The method is based on single-radial diffusion zone size enhancement (ZE) using autoradiography to demonstrate antigen--antibody reactions. The ZE technique is capable of reliably assaying concentrations of haemagglutinin as low as 0.1 microgram/ml, which represents a 20- to 40-fold increase in sensitivity over conventional single-radial-diffusion techniques. The ZE response is dependent upon the antigenic identity of the 125I-labelled 'marker' HA and the HA antigen being assayed. Haemagglutinins showing variation within a subtype (e.g. H3) show reduced ZE responses and thus the method has potential value for distinguishing between closely related HA antigens.
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