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  • Title: Loss of effectiveness of preservative systems of mascaras with age.
    Author: Bhadauria R, Ahearn DG.
    Journal: Appl Environ Microbiol; 1980 Mar; 39(3):665-7. PubMed ID: 6770758.
    Abstract:
    The preservative systems of unused, anhydrous mascaras were challenged periodically with microorganisms, using a modified membrane procedure. Shelf life of preservative activity against Pseudomonas aeruginosa varied for different brands from as little as 1 month to over 36 months. Generally, P. aeruginosa grew in mascaras after the mascaras were challenged first with Staphylococcus epidermidis or Candida albicans.
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