These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Evidence from rat liver nuclear preparations that latency of microsomal UDP-glucuronosyltransferase is associated with vesiculation.
    Author: Wishart GJ, Fry DJ.
    Journal: Biochem J; 1980 Mar 15; 186(3):687-91. PubMed ID: 6772155.
    Abstract:
    1. Nuclear, nuclear-envelope and microsomal preparations were prepared from rat liver, and their purity and morphology monitored by electron microscopy. 2. UDP-glucuronosyltransferase activity in microsomal preparations, but not in standard nuclear or nuclear-envelope preparations, displays latency from the criterion of being enhanced ('activated') by a range of detergents or the endogenous activator UDP-N-acetyl-glucosamine. 3. Nuclear preparations resemble activated rather than native microsomal preparations in failing to transfer glucuronic acid from 4-nitrophenyl glucuronide to 2-aminophenol. 4. Electron microscopy indicates that membranes of nuclear preparations and of our standard nuclear-envelope preparations remain, as in vivo, in a cisternal arrangement, whereas those of microsomal preparations are vesiculated. 5. In nuclear-envelope preparations in which vesiculation has been encouraged, the transferase can be activated by detergents. 6. We suggest that latency of UDP-glucuronosyltransferase results from vesiculation of membranes during preparation and that the latency of the microsomal transferase is largely a preparative artefact.
    [Abstract] [Full Text] [Related] [New Search]