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  • Title: [Fluorometric trihydroxyindolic method for determining catecholamines and 3,4-dihydroxyphenylalanine in urine].
    Author: Pozdeev VK.
    Journal: Biull Eksp Biol Med; 1980 May; 89(5):628-30. PubMed ID: 6772258.
    Abstract:
    Adrenalin, noradrenalin, 3,4-dioxyphenylalanine and dopamine were determined in a urine sample. Optimization of the method was reached as a result of 1) the elution procedure that combines the advantages of both the column and tube varieties; 2) standardization of the eluate and buffer concentrations, enabling necessary oxidation pH to be obtained rapidly; 3) ionic content of the buffers that intensify fluorescence of the substances under test; 4) conditions of preparing and stabilizing luteins; 5) concurret determination of noradrenalin and dopamine.
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