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  • Title: Enzyme-linked protein A: an enzyme-linked immunosorbent assay reagent for detecting antibodies in tuberculous exotic animals.
    Author: Thoen CO, Mills K, Hopkins MP.
    Journal: Am J Vet Res; 1980 May; 41(5):833-5. PubMed ID: 6773451.
    Abstract:
    An enzyme-linked immunosorbent assay (ELISA) was developed, using protein A labeled with horseradish peroxidase for detecting antibodies in tuberculous exotic mammals (llamas, rhinoceroses, elephants). The modified ELISA provides a rapid procedure for screening several animal species simultaneously for tuberculosis without the production of specific anti-species conjugates. Heat-killed cells of Mycobacterium bovis and M avium and purifed protein-derivative tuberculin of M bovis were used as antigens for ELISA.
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