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  • Title: Determination of the number of active saprophytic aquatic bacteria by semi-continuous culture on membrane filters.
    Author: Kunicka-Goldfinger W, Stronkowska E.
    Journal: Acta Microbiol Pol; 1977; 26(2):199-205. PubMed ID: 67768.
    Abstract:
    The number of bacteria in the hypolimnion waters of Lake Mikpłajski was examined by direct count on membrane filters, by the agar plate method and by semi-continuous culture on membrane filters, as previously proposed by the author. The agar plate method recovered only 20--25% of the bacteria found by direct method. On the other hand, semi-continuous culture on membrane filters allowed the recovery of about 90% of the total count. At the same time it differentiated between active, microcolony forming cells (about 70%) and inactive single cells (about 20% of the total count).
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