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  • Title: A rapid enzymic procedure for the determination of picomole amounts of UDP-glucuronic acid.
    Author: Singh J, Schwarz LR, Wiebel FJ.
    Journal: Biochem J; 1980 Aug 01; 189(2):369-72. PubMed ID: 6779812.
    Abstract:
    A simple microassay for the determination of UDP-glucuronic acid was developed on the basis of the formation of benzo[a]pyrene 3-glucuronide catalysed by UDP-glucuronyltransferase of guinea-pig liver. As little as 1-5 pmol of UDP-glucuronic acid was detectable in extracts of heat-denatured probes of liver or cultured cells equivalent to 10-50 micrograms of cellular protein.
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