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  • Title: [Continuous detection of the release of acetylcholine from the electric organ of Torpedo using a chemiluminescence reaction].
    Author: Israël M, Lesbats B.
    Journal: C R Seances Acad Sci D; 1980 Oct 27; 291(8):713-6. PubMed ID: 6780228.
    Abstract:
    A chemiluminescent procedure to measure acetylcholine was developed and used to detect continuously the release of transmitter from stimulated electric organ slices and synaptosomes. Acetylcholine is hydrolysed by acetylcholinesterase; the choline liberated is oxidized by choline oxidase to betaïne; the H2O2 generated triggers the luminescence of luminol in presence of peroxidase. The extension to other transmitters is possible; several developments are envisaged.
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