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  • Title: Inhibitory effect of gonadotropin releasing hormone upon cultured testicular cells.
    Author: Hsueh AJ, Schreiber JR, Erickson GF.
    Journal: Mol Cell Endocrinol; 1981 Jan; 21(1):43-9. PubMed ID: 6781950.
    Abstract:
    The direct inhibitory of gonadotropin-releasing hormone (GnRH) upon testicular androgen production was studied in cultured testicular cells. Enzyme-dispersed testicular cells from immature hypophysectomized rats were cultured for 6 days in a serum-free medium with or without various hormones. Culture media were changed every 2 days and media concentration of a androgens was measured by radioimmunoassay. The testis cultures from immature rats secrete predominately 5 alpha-androstane-3 alpha, 17 beta-diol (A-diol) and androsterone but negligible amounts of androstenedione, testosterone and dihydrotestosterone. Incubation of testicular cells with hCG and FSH increased A-diol and androsterone production as compared to control cultures. In contrast, treatment with GnRH or a GnRH agonist (des-Gly10, D-Leu6 (N alpha Me)Leu7, Pro9NHEt-GnRH) inhibited the gonadotropin effect in a dose-dependent manner; 10(-8) M GnRH inhibited A-diol production by approximately 20% on day 4 and 6 of culture whereas 10(-6) M GnRH inhibited A-diol production by greater than 80%. The observed effect of GnRH upon testicular cells in primary culture demonstrates that the hypothalamic peptide directly inhibits testicular steroidogenesis.
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