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  • Title: Enzyme-linked immunosorbent assay for detecting antibodies to Brucella abortus in bovine milk and serum.
    Author: Heck FC, Williams JD, Pruett J, Sanders R, Zink DL.
    Journal: Am J Vet Res; 1980 Dec; 41(12):2082-4. PubMed ID: 6782920.
    Abstract:
    An enzyme-linked immunosorbent assay (ELISA) method was evaluated for the detection of antibodies to Brucella abortus in cows milk. Milk samples from seropositive or -negative cows were sed to determine the distribution of absorbance values to classify milk as ELISA positive or ELISA negative. Brucella abortus was isolated from milk samples from 10 (45%) of the 22 cows whose milk and serum were ELISA positive. The ELISA was evaluated and determined to be an appropriate method for detecting antibodies to B abortus in bovine milk.
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