These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Peptic peptide of thiolsubtilisin. Analytical evidence for the chemical transformation of the essential serine-221 to cysteine-221.
    Author: László P, Mihály S.
    Journal: Biochim Biophys Acta; 1981 Feb 27; 667(2):351-4. PubMed ID: 6783118.
    Abstract:
    Direct evidence is offered to show that the active site Ser-221 of subtilisin is transformed into a cysteine residue in the chemical procedure previously elaborated for the preparation of thiolsubtilisin. Thiolsubtilisin was digested with pepsin and the hydrolyzate was applied to an agarose-mercurial column. After elution with 2-mercaptoethanol a single tetrapeptide was obtained. Dansyl-Edman degradation showed that the SH-peptide conformed to the amino acid sequence around the active site Ser-221 of subtilisin. A simple, single-step procedure for isolation of SH-peptides is also described.
    [Abstract] [Full Text] [Related] [New Search]