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  • Title: [Effect of 2 methods of demineralization on the on the preservation of glycoproteins and proteoglycans in the intertubular and peritubular dentin in the horse].
    Author: Goldberg M, Molon Noblot M, Septier D.
    Journal: J Biol Buccale; 1980 Dec; 8(4):315-30. PubMed ID: 6783643.
    Abstract:
    The effect of 2 methods of demineralization on the preservation of proteoglycans and glycoproteins was studied in the intertubular and peritubular dentine of the horse. The specimens embedded in Epon were demineralized with a 2% acid formic solution (Bonucci and Gheradi, 1975). Other fragments were treated with an organic solution of EDTA alkylammonium salt (Scott and Kyffin, 1979). These methods preserved in a satisfactory way these labile organic components. In the intertubular dentine, glycoproteins and proteoglycans were also identified, either associated with collagen fibres as a glue and granules, or present in the interfibrillar spaces. In the peritubular dentine, amorphous material formed a fine network between the mineral structures.
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