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  • Title: A rapid paper-disc test for penicillinase.
    Author: Tu KK, Jorgensen JH, Stratton CW.
    Journal: Am J Clin Pathol; 1981 Apr; 75(4):557-9. PubMed ID: 6784570.
    Abstract:
    A practical acidimetric assay for penicillinase production using penicillin and phenol red impregnated in paper discs was developed. Blank discs were first impregnated with buffered penicillin G and, after drying, with 1% phenol red. For use, a disc was added to a bacterial suspension made in saline solution. A yellow color within 1 to 30 min of incubation indicated penicillinase production. These discs were tested against Staphylococcus aureus, Haemophilus influenzae, and Neisseria gonorrhoeae, and the assay was found to be a simple, rapid, and accurate method for detection of penicillinase.
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