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  • Title: Release of colicin E2 from Escherichia coli.
    Author: Pugsley AP, Rosenbusch JP.
    Journal: J Bacteriol; 1981 Jul; 147(1):186-92. PubMed ID: 6787024.
    Abstract:
    Treatment of Escherichia coli K-12(ColE2.P9) with 500 ng of mitomycin C per ml resulted in rapid and almost synchronous colicin E2 production. Colicin accumulated outside the cytoplasmic membrane, most probably in the periplasmic space. Colicin release occurred during a period in which the turbidity of the culture declined markedly. Periplasmic alkaline phosphatase was released during the same period, but cytoplasmic beta-galactosidase release was delayed.
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