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  • Title: [Concentration and purification of alkaline proteinase by ultrafiltration].
    Author: Shishkova EA, Fokina SS, Trefilov EM, Fedorenko BN, Gorenkova IM.
    Journal: Prikl Biokhim Mikrobiol; 1981; 17(2):233-7. PubMed ID: 6787579.
    Abstract:
    The mode of ultrafiltration of the culture fluid of Bacillus subtilis, producer of alkaline proteinase, through semipermeable acetate cellulose membranes was examined. The ultrafiltration of the enzyme-containing solutions involved both concentration of the solution and enzyme purification from low molecular weight admixtures. The optimum mode of ultrafiltration of alkaline proteinase on UAM membranes "Vladipore" was developed.
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