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  • Title: Artificial infection of Rhipicephalus appendiculatus with Theileria parva by percutaneous injection.
    Author: Jongejan F, Perié NM, Franssen FF, Uilenberg G.
    Journal: Res Vet Sci; 1980 Nov; 29(3):320-4. PubMed ID: 6789418.
    Abstract:
    Rhipicephalus appendiculatus nymphs were inoculated with fresh or cryopreserved blood containing Theileria parva piroplasms, or with cell culture grown stages of T parva. The use of fresh blood was successful. Cryopreserved blood containing dimethylsulphoxide (DMSO), killed most nymphs after inoculation: DMSO could be removed by slow dialysis, without destroying the infectivity of the blood. Attempts to infect ticks by inoculating cell culture grown stages of T parva failed, even when large numbers of merozoites were present in the inoculum.
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