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  • Title: [Accuracy of HDL-cholesterol and HDL-apolipoprotein A-I determinations after phosphotungstate/MgCl2-precipitation of apolipoprotein B-containing lipoproteins (author's transl)].
    Author: Assmann G, Schriewer H, Funke H.
    Journal: J Clin Chem Clin Biochem; 1981 May; 19(5):273-8. PubMed ID: 6790659.
    Abstract:
    With optimal concentrations of reagents a complete precipitation of apolipoprotein B-containing lipoproteins and only a small coprecipitation of HDL can be achieved using the phosphotungstate/MgCl2 precipitation method. The accuracy of the precipitation is not influenced by triglyceride concentrations less than 4.0 mmol/l. Similarly, in most hypertriglyceridaemic sera (greater than 4.0 mmol/l) a complete precipitation of apolipoprotein B-containing lipoproteins and a complete recovery of HDL can be observed. In addition to the HDL cholesterol determination, apolipoprotein A-I was determined by kinetic nephelometry. In hypertriglyceridaemic sera (greater than 4.56 mmol/l) precipitation of apolipoprotein B-containing lipoproteins must be carried out prior to the nephelometric determination of apolipoprotein A-I.
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