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  • Title: Preparation of anti-Gm sera by immunization of rabbits with protein A-fractionated normal IgG proteins from Japanese: further study.
    Author: Tamaki Y, Kishida T, Shibata K, Takahashi N, Fukuda M.
    Journal: J Immunol Methods; 1981; 45(2):177-82. PubMed ID: 6793663.
    Abstract:
    A method is described for the production of rabbit anti-Gm sera using normal IgG proteins and fragments thereof as the immunogens. Anti-G3m(16) and anti-G3m(21) were readily producible by immunizations with protein A-purified IgG3 proteins of G3m(13, 15, 16) and G3m(21) type, respectively. Anti-G1m(2), anti-G1m(3), and anti-G3m(5) were obtained by immunizations with type Gm(1, 2, 21) Fc fragments, type Gm(1, 3, 5, 13) IgG1-rich IgG, and type G3m(5, 13, 21) IgG3, respectively. These immune sera can distinguish the 9 Gm phenotypes in the Japanese.
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