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  • Title: Preparation and partial characterization of cell-free protein-synthesizing extracts from Tetrahymena pyriformis.
    Author: David ET, Smith KE.
    Journal: Biochem J; 1981 Mar 15; 194(3):761-70. PubMed ID: 6796065.
    Abstract:
    1. We have examined methods necessary for preparing post-mitochondrial supernatants from Tetrahymena pyriformis strain HSM that are capable of efficient cell-free protein synthesis. 2. The requirements for optimum synthesis in these extracts are described. 3. Data relating to the kinetics of protein synthesis and the initiation capacity of these supernatants are presented.
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