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  • Title: Amino acid transport in rat Sertoli cell enriched testes: studies on the mechanism of action of follicle stimulating hormone.
    Author: Pérez-Sánches VH, Wassermann GF.
    Journal: Braz J Med Biol Res; 1981 Apr; 14(1):11-7. PubMed ID: 6796160.
    Abstract:
    1. The in vivo administration of ovine Follicle Stimulating Hormone (FSH) (15 mu g/rat, 1 h before measurements) enhanced the transport of Alpha [3-14C]-aminoisobutyric acid ([14C]-AIB) in Sertoli cell enriched testes of 10, 15 and 20-day-old rats. The effect was not observed in 5 or 25-day-old rats. 2. Increasing the intracellular concentration of cyclic AMP by means s cholera toxin, exogenous cyclic AMP or 1-methyl-3-isobutylxanthine did not change either the control or the FSH-stimulated uptake of [14C]-AIB. 3. The inhibition of glycoprotein synthesis by tunicamycin decreased the uptake of [14C]-AIB in controls and significantly reduced (24%) the effect of FSH on [14C]-AIB transport in Sertoli cell enriched testes.
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