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  • Title: Switch from NP-specific IgG3 to IgG1 in the mouse hybridoma cell line S24/63/63.
    Author: Baumhäckel H, Liesegang B, Radbruch A, Rajewsky K, Sablitzky F.
    Journal: J Immunol; 1982 Mar; 128(3):1217-20. PubMed ID: 6799571.
    Abstract:
    We have isolated by fluorescence-activated cell sorting an IgG1 expressing class switch variant (S24-1/47) from the IgG3-producing mouse hybridoma cell line S24/63/63. Both Ig bind the hapten 4-hydroxy-3-nitro-phenylacetyl (NP) with approximately the same affinity and fine specificity and are idiotypically indistinguishable. The apparent m.w. is 50,000 for the gamma 1 and 51,000 for the gamma 3 chain. The genomic DNA of IgG3-producing S24/63/63 cells contains a 14-kb Eco RI restriction fragment with C gamma 3 sequences representing a rearranged C gamma 3 gene. In the class switch variant S24-1/47 the C gamma 3 gene is deleted.
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