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  • Title: Expression of surface membrane IgG on pokeweed mitogen-reactive anti-tetanus toxoid antibody-producing cells.
    Author: Thiele CJ, Stevens RH.
    Journal: J Clin Immunol; 1981 Jul; 1(3):174-80. PubMed ID: 6801082.
    Abstract:
    Anti-tetanus toxoid antibody-producing cells, differentially expressing surface membrane IgM, were analyzed for the additional expression of surface membrane IgG. micron+ and micron- cells were rosetted with anti-gamma-ox red blood cells and separated by density centrifugation into fractions enriched or depleted of gamma + cells. These B-cell subsets were assayed for the production of IgM and IgG anti-tetanus toxoid antibody and total IgM and IgG. The results indicated that the majority of anti-tetanus toxoid antibody synthesis in the micron- fraction was by gamma + cells. In the microm+ fraction, however, both IgM and IgG anti-tetanus toxoid antibody production was detected in the micron+ gamma- and micron+ gamma+ fraction. The inclusion of isotype-specific antisera during the first 2 days of culture further established that gamma was expressed on the surface of the majority of the precursors for IgG anti-tetanus antibody production in vitro. Studies performed to determine the culture requirements of micron- and micron+ cells revealed that production of IgG anti-tetanus toxoid antibody by both cell subsets was dependent on T cells and pokeweed mitogen. However, some micron- cells could produce IgG in the presence of T cells alone.
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