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  • Title: [Production of monospecific type A botulinum toxin and antiserum using a column chromatographic method].
    Author: Marchev N, Marcheva D.
    Journal: Vet Med Nauki; 1982; 19(1):57-63. PubMed ID: 6810534.
    Abstract:
    A column chromatography method was employed to obtain a monospecific botulinus type A toxin. The latter was found to be a homogeneous product via column chromatographic and electrophoretic studies. The immunodiffusion test after Ouchterlony with a serum obtained with the use of a native toxin yielded one precipitation line. No such lines were produced with a serum obtained from animals immunized with a type B toxin. The purified toxin could be detoxicated by routinely applied methods. When injected to rabbits it induced the production of monospecific antibodies.
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