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  • Title: The interaction of ram proacrosin and acrosin forms with antiserum raised against ram m beta-acrosin.
    Author: Harrison RA.
    Journal: J Reprod Immunol; 1982 Aug; 4(4):231-49. PubMed ID: 6813463.
    Abstract:
    An antiserum was raised in rabbits to pure m beta-acrosin, the stable form of active acrosin from ram spermatozoa. By use of immunoprecipitation with Protein A-bearing Staphylococcus aureus cells, so as to react antigen with antibody and isolate the complex rapidly, it could be demonstrated that the anti-m beta-acrosin cross-reacted strongly with native proacrosin, with a degenerate proacrosin form, and with at least one form of active acrosin other than m beta-acrosin. Sperm proteins unrelated to acrosin were not recognized. It is concluded that rabbit anti-m beta-acrosin will react specifically with all the major ram acrosin forms; the antiserum can therefore be used with confidence in immunocytochemical studies to locate both proacrosin and acrosin in ram spermatozoa.
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