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  • Title: Bromodeoxyuridine-induced molecular species conversion of sialic acids of gangliosides and the alteration of cellular phenotypic expression in B16 mouse melanoma cells.
    Author: Kinoshita Y, Makita A, Takeuchi T.
    Journal: J Biochem; 1982 Sep; 92(3):801-8. PubMed ID: 6815168.
    Abstract:
    Gangliosides of B16 mouse melanoma cells were characterized and the effects of 5-bromodeoxyuridine (BrdU) on gangliosides were examined in relation to the alteration of cellular phenotypic expression. In melanotic cells, gangliosides were shown to be composed of both hematosides containing N-acetylneuraminic acid (NeuAc-hematoside) and N-glycolylneuraminic acid (NeuGc-hematoside) as determined by thin-layer chromatography, gas chromatography-mass spectrometry and the use of anti-NeuGc-hematoside antiserum. Gangliosides other than hematosides were not detectable under the conditions of thin-layer chromatography employed. BrdU treatment of the melanotic cells induced about a two-fold increase in the ratio of NeuGc-hematosides to the total hematosides without any significant change in the cellular ganglioside content. The treatment concomitantly induced a change in cellular morphology, an increase in cell-to-substrate adhesiveness, and the suppression of tyrosinase activity. Amelanotic cells, which showed cellular phenotypes similar to those of BrdU-treated melanotic cells, were demonstrated to have over twice as much NeuGc-hematosides as compared with the melanotic cells. These results indicate a possible relationship in melanoma cells between the molecular species conversion of sialic acids of hematosides and the alteration of cellular phenotypic expression by BrdU treatment or spontaneous amelanization.
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