These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Granuloma pouch assay. IV. Induction of sister-chromatid exchanges in vivo.
    Author: Maier P, Frei K, Weibel B, Zbinden G.
    Journal: Mutat Res; 1982 Oct; 97(5):349-57. PubMed ID: 6815527.
    Abstract:
    SCEs were induced, in vivo, in cells of a rapidly proliferating subcutaneous granulation tissue, initiated by the formation of a subcutaneous air pouch, on the backs of adult male rats (Granuloma Pouch Assay). 2 days after pouch formation the test compounds were applied, and 24 h later the granulation tissue was excised and dissociated into single cells. Isolated cells were cultured in vitro in media containing BrdU, and SCEs were determined within 24-48 h. The spontaneous frequency was 14.4 +/- 1.1 per metaphase. Mitomycin C (MMC) and cyclophosphamide (CP) induced a significant and dose-dependent increase in SCE frequencies. Results obtained after i.v., i.p. and intra-pouch application routes are compared.
    [Abstract] [Full Text] [Related] [New Search]