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  • Title: [Carboxyl groups in the active center of glucoamylase from Aspergillus awamori].
    Author: Savel'ev AN, Firsov LM.
    Journal: Biokhimiia; 1982 Oct; 47(10):1618-20. PubMed ID: 6816298.
    Abstract:
    The values for the ionization constants of the catalytic groups of the active site of glucoamylase from Asp. awamori for the free enzyme and for the enzyme--substrate complex were calculated. The temperature dependence of the alkaline branch of the pH-dependence curve and the pH dependence in the presence of methanol were determined. The ionization enthalpy delta H = 1.5 +/- 0.3 kcal/mole, the ionization entropy delta S = 20.5 +/- 1.2 e. u. It was assumed that two carboxyl groups are involved in the catalytic act.
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