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  • Title: Facile in vivo transfer of mutations between the Bacillus subtilis chromosome and a plasmid harbouring homologous DNA.
    Author: Chak KF, de Lencastre H, Liu HM, Piggot PJ.
    Journal: J Gen Microbiol; 1982 Nov; 128(11):2813-6. PubMed ID: 6818329.
    Abstract:
    Transformation of Bacillus subtilis strains carrying the point mutations spoIIA42 or spoIIA69 by a plasmid, pHM2, carrying the spoIIA+ allele gave occasional recombinants in which the plasmid had acquired the chromosomal mutation. Transformation of a spo+ strain by these derivative plasmids gave occasional recombinants in which the chromosome of the recipient had acquired the plasmid-borne spoIIA mutation. Both types of behaviour were recE+-dependent. Mutations spoIIA42 and spoIIA69 fell into a single complementation group.
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