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  • Title: Preparation of type-specific antimyosin antibodies and determination of their specificity by biochemical and immunohistochemical methods.
    Author: Carpenè E, Rowlerson A, Veggetti A, Mascarello F.
    Journal: Ital J Biochem; 1982; 31(5):329-41. PubMed ID: 6820361.
    Abstract:
    This paper reports the preparation of specific anti-slow myosin antibodies (anti-I) and anti-fast myosin antibodies (anti-IIA) raised against myosins from sheep and guinea pig masseter muscles. The specificity of the antibodies has been studied by immunodiffusion in agar and by the GEDELISA test using slow-twitch (type I), fast-twitch red (type IIA) and fast-twitch white (type IIB) myofibrils isolated from guinea pig muscles. The principal specificity of the anti-I and anti-IIA antibodies was for the heavy chains of type I and IIA myosins, respectively. A smaller reaction with the corresponding light chains was also detected. Immunohistochemical staining of muscle sections using these antibodies confirmed their fibre type specificity.
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