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  • Title: [Immunocytochemical demonstration of neuropeptides (neurophysin and oxytocin) by the protein A-gold technic].
    Author: Lösecke W, Naumann W, Sterba G.
    Journal: Acta Histochem; 1982; 71(2):201-8. PubMed ID: 6820602.
    Abstract:
    In the classic peptidergic neurosecretory system of vertebrates the neurohormone oxytocin is associated with the carrier protein neurophysin. By using small gold particles (3 to 12 nm) in the protein A-gold technique, both the hormone and the carrier-protein will be labelled, while larger particles (20 nm) label only the neurophysin. To bind the latter particles, a greater number of Fc-regions of antigen-IgG-complexes seems to be necessary.
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