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  • Title: The effect of an integral membrane protein on lipid polymorphism in the cardiolipin-Ca2+ system.
    Author: Taraschi TF, de Kruijff B, Verkleij AJ.
    Journal: Eur J Biochem; 1983 Jan 01; 129(3):621-5. PubMed ID: 6825677.
    Abstract:
    The addition of Ca2+ to aqueous dispersions of cardiolipin triggers complete hexagonal (HII) phase formation at Ca2+/cardiolipin molar ratios greater than or equal to 1.0 as detected by 31PNMR and freeze-fracture electron microscopy. Incorporation of the integral membrane protein glycophorin prevents the bilayer leads to hexagonal (HII) phase transition at Ca2+/cardiolipin ratios as high as 15:1. Removal of the outwardly oriented, negatively charged sialic-acid-containing sugar groups of glycophorin with trypsin had little effect on the bilayer-stabilizing capacity of the protein. As the Ca2+ binding was found to be similar in both the cardiolipin and the cardiolipin-glycophorin systems, it can be concluded that the protein exerts a bilayer-stabilizing effect on the cardiolipin. In addition, the possibility that glycophorin may prevent vesicle fusion is also discussed.
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