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  • Title: Beef liver glutamate dehydrogenase: effects of partial proteolysis with chymotrypsin.
    Author: Aitchison MJ, Engel PC.
    Journal: Int J Biochem; 1983; 15(1):79-85. PubMed ID: 6825906.
    Abstract:
    1. After chymotryptic digestion of bovine glutamate dehydrogenase, the assay conditions determine whether activation or inhibition is observed. 2. The major fragments appear to remain physically associated. 3. Responses to both GTP and ADP are altered. Inhibition by GTP at pH 7 and 8 is almost abolished. 4. Out of various ligand combinations tested, GTP and NADH together provide the best protection against all the proteolytic effects.
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