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  • Title: The labelling of an axonal membrane component with 4-(N-maleimido) benzyltrimethylammonium, a reagent capable of affinity-labelling the alpha-subunit of the nicotinic acetylcholine receptor.
    Author: Mautner HG, Cole JL, Belew MA.
    Journal: Biochem Biophys Res Commun; 1983 Feb 28; 111(1):61-6. PubMed ID: 6830602.
    Abstract:
    Membrane vesicles, isolated from crustacean axons, were treated, following disulfide reduction, with 3H-NEM or with 3H-MBTA. SDS polyacrylamide gel electrophoresis showed that exposure to NEM (a nonspecific thiol reagent) resulted in the labelling of several peptide bands, while with MBTA only a single band with a molecular weight of 50,000 was labelled. Reaction with MBTA (believed to be a specific label for the nicotinic acetylcholine receptor) could be largely prevented by preincubation with d-tubocurarine or bromacetylcholine.
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