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  • Title: Reversible effects of cross-linking on the regulatory cooperativity of Acinetobacter citrate synthase.
    Author: Mitchell CG, Weitzman PD.
    Journal: FEBS Lett; 1983 Jan 24; 151(2):260-4. PubMed ID: 6832355.
    Abstract:
    Citrate synthase was purified from Acinetobacter calcoaceticus and treated with the cleavable cross-linking reagent dithiobis(succinimidyl propionate). Cross-linking of the enzyme resulted in the abolition of the sigmoidal responses to inhibition by NADH and re-activation by AMP displayed by the native enzyme. Inhibition and re-activation were still observed but without any cooperativity. Cleavage of the disulphide bonds in the cross-links by treatment with dithiothreitol restored the sigmoidal characteristics of both inhibition and re-activation.
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