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  • Title: Replication of influenza A and B viruses in human diploid cells.
    Author: Herrero-Uribe L, Mann GF, Zuckerman AJ, Hockley D, Oxford JS.
    Journal: J Gen Virol; 1983 Feb; 64 (Pt 2)():471-5. PubMed ID: 6834005.
    Abstract:
    Under optimal conditions, of high multiplicities of infection and with trypsin included in the medium throughout the incubation period, high yields of infectious influenza A and B viruses (10(6 . 5) p.f.u./ml) and of antigenically active haemagglutinin (HA)(1 microgram/HA/10(6) cells) were produced in human diploid MRC-5 cells. Budding virus particles were seen as spherical or short rod-like protrusions on the surface of the infected cells, and also on cell filopodia. Virus-induced cytoplasmic and nuclear inclusions were present in infected cells. This virus-human cell system may be suitable for studies of influenza virus persistence and for production of immunologically active HA antigen.
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