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  • Title: Biosynthesis and processing of placental alkaline phosphatase.
    Author: Ito F, Chou JY.
    Journal: Biochem Biophys Res Commun; 1983 Mar 16; 111(2):611-8. PubMed ID: 6838575.
    Abstract:
    Polypeptides of 61,500 and 64,500 apparent molecular weights were the precursor and fully processed forms of placental alkaline phosphatase monomer synthesized by choriocarcinoma cells in vivo. [3H] Mannose was incorporated into both polypeptides whereas [3H] glucosamine was incorporated mainly into the 64,500-dalton polypeptide, suggesting processing by the addition of glucosamine moieties. In the absence of microsomal membranes, choriocarcinoma mRNA directed the cell-free synthesis of the preprotein form of alkaline phosphatase monomer of apparent Mr = 60,000. The unglycosylated monomer had an apparent Mr = 58,000. In the presence of microsomal membranes, the 60,000-dalton polypeptide was processed to a polypeptide of apparent Mr = 61,500, comigrating with the precursor form of alkaline phosphatase monomer.
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