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  • Title: In vitro metabolism of estradiol-17 beta by liver microsomes from juvenile rainbow trout, Salmo gairdneri.
    Author: Hansson T, Rafter J.
    Journal: Gen Comp Endocrinol; 1983 Mar; 49(3):490-5. PubMed ID: 6840534.
    Abstract:
    The metabolism of [4-14C]estradiol-17 beta by liver microsomes from, Salmo gairdneri, was studied in vitro. The following metabolites were identified by gas chromatography--mass spectrometry: estrone, estra-1,3,5(10)-triene-3,16 beta,17 beta-triol, and estra-1,3,5(10)-triene-3,7 alpha,17 beta-triol. The presence of additional polar metabolites hydroxylated at positions 6 alpha and 6 beta was indicated. The formation of the hydroxylated metabolites was inhibited by CO indicating the participation of cytochrome P-450-dependent enzymes.
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