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  • Title: Properties of narrow-range ampholytes isolated from wide-range ampholyte preparations.
    Author: Binion SB, Rodkey LS, Egen NB, Bier M.
    Journal: Anal Biochem; 1983 Jan; 128(1):71-6. PubMed ID: 6846801.
    Abstract:
    A simple procedure for obtaining useful narrow-pH-range ampholytes from inexpensive laboratory-synthesized ampholytes by preparative isoelectric focusing in Pevikon is described. The narrow-range ampholytes prepared in this way are comparable to commercial ampholyte preparations as judged by conductivity, buffer capacity, pH gradient formation, and resolving power. These inexpensive narrow-range ampholytes are particularly well suited to preparative isoelectric focusing applications requiring large quantities of ampholytes.
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