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  • Title: Specific and rapid assay of urinary oxalic acid using high-performance liquid chromatography.
    Author: Imaoka S, Funae Y, Sugimoto T, Hayahara N, Maekawa M.
    Journal: Anal Biochem; 1983 Feb 01; 128(2):459-64. PubMed ID: 6846821.
    Abstract:
    A high-performance liquid chromatographic (HPLC) method was developed to determine the levels of oxalic acid in the urine. This acid was extracted from urine with tri-n-butyl phosphate and converted into the fluorescent derivative by esterification with 9-anthryldiazomethane (ADAM). The reaction mixture containing the oxalic acid derivative can be directly chromatographed on HPLC using octadecylsilane reverse-phase column monitoring with a fluorophotometric detector. A linear relationship was observed in the range from 1 to 100 micrograms/ml of standard oxalic acid dissolved in saline. Disease-free Japanese adults excrete 23.8 +/- 9.0 mg (mean +/- SD) of oxalic acid per day. This method should prove valuable for routine measurements of urinary oxalic acid as it is accurate, simple, and specific.
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