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  • Title: A simple assay to study protein-mediated lipid exchange by fluorescence polarization.
    Author: Xü YH, Gietzen K, Galla HJ, Sackmann E.
    Journal: Biochem J; 1983 Jan 01; 209(1):257-60. PubMed ID: 6847612.
    Abstract:
    We investigated the protein-mediated phospholipid transfer between small vesicles by fluorescence polarization measurements with diphenylhexatriene as optical probe. Thermotropic-phase-transition curves were taken after mixing two vesicle preparations of lipids exhibiting different gel-to-liquid phase transitions. From the heights of each phase-transition step we were able to follow the lipid transfer process without separating the two vesicle preparations.
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