These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


PUBMED FOR HANDHELDS

Search MEDLINE/PubMed


  • Title: Kinetics of conformational transitions of demetalized Concanavalin A.
    Author: Brewer CF, Brown RD, Koenig SH.
    Journal: Biochem Biophys Res Commun; 1983 Apr 29; 112(2):595-601. PubMed ID: 6847668.
    Abstract:
    Demetalized Concanavalin A exists in two conformational states, known as locked (PL) and unlocked (P) [Brown et al., Biochemistry 16, 3883 (1977)]. The equilibrium ratio [PL]/[P] is 0.14 +/- 0.01 at 25 degrees C, pH 6.4 [Brown et al., Biochemistry 21, 465 (1982)]. We now report values of the rate constants for the P in equilibrium; k1 = (33 +/- 4 h)-1 and k-1 = (4.6 +/- 0.6 h)-1 for the P leads to PL and PL leads to P transitions, respectively, at 25 degrees C, pH 6.4. The experiments utilize the fact that saccharide binds to PL [Koenig et al., Biochemistry 17, 4251 (1978)], producing a time-dependent increase in the total concentration of locked forms at equilibrium, and use a new technique for measuring this concentration.
    [Abstract] [Full Text] [Related] [New Search]