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  • Title: Influence of bilirubin on the determination of acid phosphatase in serum.
    Author: Hoffmann GE, Weiss L.
    Journal: J Clin Chem Clin Biochem; 1983 Jan; 21(1):31-3. PubMed ID: 6854218.
    Abstract:
    Bilirubin interferes greatly in the continuous measurement of acid phosphatase, using alpha-naphthyl phosphate as substrate and diazotized 2-amino-5-chlorotoluene (Fast Red TR) as the colorimetric reagent. Fast Red TR and the so-called "direct" (but not "indirect") bilirubin form a coloured azo-compound which absorbs light above 480 nm. Negative absorbance changes are observed at 405 nm, which is used in the determination of acid phosphatase. This leads to apparently negative activities.
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