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  • Title: Rapid and reversible inhibition of junctional communication by tumor promoters in a mouse cell line.
    Author: Fitzgerald DJ, Knowles SE, Ballard FJ, Murray AW.
    Journal: Cancer Res; 1983 Aug; 43(8):3614-8. PubMed ID: 6861133.
    Abstract:
    HEL-37 cells rapidly transferred microinjected fluorescein between contacting cells. This transfer was strongly inhibited by tumor-promoting phorbol esters and mezerein but not by non-promoting derivatives. The onset of the inhibition occurred after 5 to 10 min of exposure to the test compound and was complete within 20 min. Using a wash-off procedure, it was demonstrated that inhibition of dye transfer by phorbol-12, 13-dibutyrate was fully reversed by 100 min, after a lag of 40 to 60 min. The recovery of dye transfer competence was blocked by puromycin. Both 12-O-tetradecanoylphorbol-13-acetate and monensin caused elevated Na+ levels in HEL-37 cells, but the latter compound did not inhibit dye transfer. It was concluded that the promoter inhibition of transfer was not a consequence of Na+-induced increase in cytoplasmic Ca2+ concentration.
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