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  • Title: Synthesis of rat-liver lactate dehydrogenase and characterization of its mRNA.
    Author: Eisenmann A, Schmelzer E, Northemann W, Phillips J, Kaiser C, Witt I, Decker K, Heinrich PC.
    Journal: Eur J Biochem; 1983 Jul 15; 134(1):39-45. PubMed ID: 6861761.
    Abstract:
    Rat liver lactate dehydrogenase (LDH) has been synthesized in hepatocytes and in a cell-free translation system. The subunit synthesized in both systems displayed the same electrophoretic mobility upon sodium dodecyl sulfate/polyacrylamide slab gel electrophoresis. Sequence analysis of the subunits translated in vitro and synthesized in vivo indicated N-acetyl-alanyl-alanine for both N termini. Thus, the newly synthesized subunit does not exhibit an amino-terminal extension. The mRNA for the lactate dehydrogenase subunit was exclusively found in free polysomes. A size of 2120 +/- 240 nucleotides was estimated for the mRNA. Only about 50% of these nucleotides are needed to code for the polypeptide chain of the enzyme.
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