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  • Title: Simultaneous determination of protein (nitrogen), phosphorus, and calcium in animal feedstuffs by multichannel flow-injection analysis.
    Author: van Staden JF.
    Journal: J Assoc Off Anal Chem; 1983 May; 66(3):718-26. PubMed ID: 6863192.
    Abstract:
    A 3-channel flow-injection procedure was developed, which enables the simultaneous determination of protein, phosphorus, and calcium in a wide range of animal feeds from a single digestion. Samples are digested with a block digestor, diluted, and analyzed at a rate of 82 samples/h. Protein (nitrogen) as ammonia is determined colorimetrically by the indophenol method. Phosphorus and calcium are determined by measuring the absorbances of the molybdenum blue and calcium-cresolphthalein complexes at 660 and 580 nm, respectively. Protein is determined in the range from 0 to 75%, phosphorus in the range from 0 to 6%, and calcium in the range from 0 to 6%. The results obtained do not differ significantly from those obtained by proven manual methods, and considerable time, space, and reagents are saved.
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